In vitro propagation of brazilian nut tree (Bonpl.)
DOI:
https://doi.org/10.54353/ritp.v3i2.e002Keywords:
brazilian nuts, in vitro culture, micropropagation, woody plant medium, establishment, multiplication, sucrose, explantsAbstract
Bertholletia excelsa, known as Brazil nut, is a forest species of great economic and ecological importance at the national and international level, currently its presence in the forests of Madre de Dios is threatened by anthropogenic activities such as deforestation, soil degradation and decline in natural populations. Considering this problem, the in vitro micropropagation of this species was carried out as a strategic technology for its conservation and large-scale production in the present work. For the micropropagation in vitro, 6 treatment were tested based in factors as 2 concentrations of Woody Plant Medium (WPM) at 50 and 100% and 3 concentrations of sucrose (30, 40 and 5, g/L), obtaining results that the best protocol corresponds to WPM at 50 % with 30 g/L of sucrose, giving a growth of 2.37 cm, 2.73 nodes and 1.60 leaves on average. Finally, we indicate that medium with WPM 50% and 30 g/L sucrose concentration is efficient for plant tissue culture to this valuable forest species.
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Anis M, Ahmad N. (2016). Plant tissue culture: propagation, conservation and crop improvement. https://doi.org/10.1007/978-981-10-1917-3
Arias, E., & Rondón, J. (2010). Manejo Forestal de Bertholletia excelsa HBK (castaña o nuez de Brasil). Revista Forestal Latinoamericana, 25(1), 93-113. https://bit.ly/3xndGLN
Alonso A, Beltrán M, Gelvez J, Martinez J. (2020). Evaluación de dos protocolos de desinfección para el establecimiento in vitro de portainjertos de aguacate (Persea americana) var. criollo. En II Simposio Nacional de Investigación Ciencias Pecuarias y Agroempresariales, La Dorada, Caldas. https://bit.ly/3KaxKbO
Borges, M.; Portales, S.; Malaurie, B. (2008). Efectos de diferentes concentraciones de sacarosa en el cultivo in vitro de coco (Cocos nucifera L.). Revista Colombiana de Biotecnología, 10(2), 111-119. https://bit.ly/3I2FaLB
Brand, M. H. (1993). Agar and ammonium nitrate influence hyperhydricity, tissue nitrate and total nitrogen content of serviceberry (Amelanchier arborea) shoots in vitro. Plant Cell, Tissue and Organ Culture, 35(3), 203-209. https://link.springer.com/article/10.1007/BF00037271
Brondani G, Hansel F, Dutra L, Wendling I. 2010. Desinfestação e meio de cultura para o estabelecimento in vitro de segmentos nodais de Liquidambar styraciflua. Floresta, 40(3): 541-554. http://dx.doi.org/10.5380/rf.v40i3.18916
Carrión A, Tapia M. 2019. Yield of five potato varieties in Temporary Immersion Bioreacctors. Peruvian Journal of Agronomy. 3, 24-28. https://doi.org/10.21704/pja.v3i1.1281
Chalupa, V. (1993). Vegetative propagation of oak (Quercus robur and Q petraea) by cutting and tissue culture. In annales des sciences forestières. 50, Supplement 1, 295s-307s. https://hal.science/hal-00882901/document
Ferro Mauricio, R. D. (2021). Conservación de oca (Oxalis tuberosa) bajo condiciones in vitro y corroboración de la estabilidad genética. [Tesis de Biólogo, Universidad Nacional Mayor de San Marcos]. http://bit.ly/3RVdJry
Figueiredo, F. J. C., & de Carvalho, C. J. R. (2002). Aspectos fisiológicos de sementes de castanha-do-brasil submetidas a condições de estresse: emergência e respiração. Boletim de Pesquisa e Desenvolvimento. https://bit.ly/3K6kIfe
Fry, J.C. (1993). Biological Data Analysis: a practical approach. Oxford University Press.
George, E. (1993). Plant Propagation by Tissue Culture. Springer.
Grout, B. W. V. (1991, April). Conservation in vitro. International Symposium on Plant Biotechnology and its Contribution to Plant Development, Multiplication and Improvement, 289, 171-178.
Hiti-Bandaralage J, Hayward A, Mitter N. 2017. Micropropagation of Avocado (Persea americana Mill.). American Journal of Plant Sciences. https://doi.org/10.4236/ajps.2017.811197
Karunarate, S. 1989. Informe sobre el cultivo de embriones del cocotero enano. En: Information Express. CIDA 13 (3).
Lloyd, G., & McCown, B. (1981). Woody Plant Medium: A mineral nutrient formulation for microculture of woody plant species. HortScience, 16, 453.
MINAM, 2014. La castaña Amazónica regalo de la biodiversidad. Sistematización de experiencias de investigación y manejo de castaña (Bertholletia exhttps://repositoriodigital.minam.gob.pe/handle/123456789/768celsa) en ecosistemas de terrazas altas en el departamento de Madre de Dios. https://repositoriodigital.minam.gob.pe/handle/123456789/768
Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia plantarum, 15(3), 473-497. https://bit.ly/3RSAe0l
OSINFOR (Organismo de Supervisión de los Recursos Forestales y de Fauna Silvestre). (2018). Aprovechamiento Forestal Maderable en Concesiones de Castaña. Lima, Perú. http://bit.ly/3K6Oqkw
Phillips, G. C., & Garda, M. (2019). Plant tissue culture media and practices: an overview. In Vitro Cellular & Developmental Biology-Plant, 55(3), 242-257.
Ramos Robles, L. M. (2018). Influencia del régimen de perturbación de los bosques con castaña en la calidad de las semillas y el vigor de las plántulas de Castaña (Bertholletia excelsa HBK) en Madre De Dios, 2018. http://repositorio.unamad.edu.pe/bitstream/handle/20.500.14070/328/004-2-3-064.pdf?sequence=1
Rache, L. & Pacheco, J. 2012. Establecimiento de un protocolo de propagación de Physalis peruviana L. a partir de yemas axilares adultas. Ciencia en Desarrollo. 4(1). https://doi.org/10.19053/01217488.477
Roca, W., & Mroginski, L. 1993. Cultivo de tejidos en la agricultura: fundamentos y aplicaciones. CIAT. Colombia. http://bit.ly/3E0Ae8E
Rockwell, C. A., Guariguata, M. R., Menton, M., Arroyo Quispe, E., Quaedvlieg, J., Warren-Thomas, E., ... & Yucra Salas, J. J. (2015). Nut production in Bertholletia excelsa across a logged forest mosaic: implications for multiple forest use. PLoS One, 10(8), e0135464. https://bit.ly/3xkeOzF
Withers, L. A. (1991). 4. In-vitro conservation. Biological Journal of the Linnean Society, 43(1), 31-42.
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Copyright (c) 2022 Henry Robles Cueva , Francisco Román-Dañobeytia, Jose Luis Rafael Quille , Cesar Enrique Álvarez Sanchez , Nelson Leopoldo Meléndez Ascaño , Renzoandré De la Peña Lavander, Olegario Robles Cueva
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Esta obra está bajo licencia internacional Creative Commons Reconocimiento 4.0 Internacional (CC BY 4.0).
La "Revista de Innovación y Transferencia Productiva" del Instituto Tecnológico de la Producción, Lima, Perú se distribuye bajo una Licencia Creative Commons Reconocimiento 4.0 Internacional (CC BY 4.0).
